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One of the criticisms received by the method refers to the fact that the VO 2 max cannot be accurately measured in either of the methods, with water-sealed spirometry or BSA based approximation.

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In this theoretical framework, diffusion is due to fluctuations whose dimensions range from the molecular scale to the macroscopic scale.

In particular, fluctuating hydrodynamic equations include a Fick's flow term, with a given diffusion coefficient, along with hydrodynamics equations and stochastic terms describing fluctuations.

When calculating the fluctuations with a perturbative approach, the zero order approximation is Fick's law. The first order gives the fluctuations, and it comes out that fluctuations contribute to diffusion.

This represents somehow a tautology , since the phenomena described by a lower order approximation is the result of a higher approximation: this problem is solved only by renormalizing the fluctuating hydrodynamics equations.

The adsorption or absorption rate of a dilute solute to a surface or interface in a gas or liquid solution can be calculated using Fick's laws of diffusion.

The accumulated number of molecules adsorbed on the surface is expressed by the Langmuir-Schaefer equation at the short-time limit by integrating the diffusion equation over time: [9].

The Langmuir-Schaefer equation is extended to the Ward-Tordai Equation to account for the back-diffusion of the rejected molecules from the surface in the later time of the adsorption: [10].

The square root t dependent on the adsorption is because when the molecules are adsorbed, the concentration in the sub-surface drops and creates a concentration gradient near the surface which slows down the absorption over time.

This assumption can be confirmed with a Monte Carlo simulation. The langmuir-Schaefer equation can also be obtained from analysing the single-molecule diffusion.

Considering one dimension that is perpendicular to the surface, the probability of any given solute molecule in the solution hit the surface is the error function of its diffusive broadening over the time of interest.

At a longer time, the Langevin equation merges into the Stokes—Einstein equation. The latter is appropriate for the condition of the diluted solution, where long-range diffusion is considered.

According to the fluctuation-dissipation theorem based on the Langevin equation in the long-time limit and when the particle is significantly denser than the surrounding fluid, the time-dependent diffusion constant is: [13].

For a single molecule such as organic molecules or biomolecules e. When the area of interest is the size of a molecule specifically, a long cylindrical molecule such as DNA , the adsorption rate equation represents the collision frequency of two molecules in a diluted solution, with one molecule a specific side and the other no steric dependence, i.

The diffusion constant need to be updated to the relative diffusion constant between two diffusing molecules.

This estimation is especially useful in studying the interaction between a small molecule and a larger molecule such as a protein.

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